Anticorps Monoclonal anti-UCP2

UCP2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2B4B1

N° de cat : 66700-1-Ig

Synonymes

BMIQ4, SLC25A8, UCP 2, UCP2, UCPH

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Galerie de données de validation

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  WB analysis of Jurkat using 66700-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adipose using 66700-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66700-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu adipeux humain, tissu de muscle squelettique humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66700-1-Ig cible UCP2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UCP2 Protéine recombinante Ag19361
• Nom complet: uncoupling protein 2 (mitochondrial, proton carrier)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC011737
• Symbole du gène: UCP2
• Identification du gène (NCBI): 7351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.