Anticorps Polyclonal de lapin anti-Titin

Titin Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (2)

N° de cat : 27867-1-AP

Synonymes

CONNECTIN, titin, TMD, TTN

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Galerie de données de validation

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  IHC staining of mouse skeletal muscle using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27867-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27867-1-AP (Titin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris, cellules HeLa, tissu cardiaque de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

27867-1-AP cible Titin dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Titin Protéine recombinante Ag27496
• Nom complet: titin
• Masse moléculaire calculée: 3816 kDa
• Numéro d’acquisition GenBank: NM_001256850
• Symbole du gène: TTN
• Identification du gène (NCBI): 7273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Elife; A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation.; Authors - Jie Wang
mouse	IF	Dev Biol; Aberrant differentiation of second heart field mesoderm prefigures cellular defects in the outflow tract in response to loss of FGF8; Authors - Sophie Astrof
human	IF	Stem Cell Res; Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes; Authors - Sun-Ho Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Víctor (Verified Customer) (01-26-2023)

The antibody is a bit dirty but specific for muscle cells. I'm trying to evaluate specific sarcomeric structures with higher magnifications, and they seem to be marked. So the antibody is OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Skeletal muscle cells obtained by direct cell reprogramming approaches

Titin Antibody Immunofluorescence validation (1:400 dilution) in Skeletal muscle cells obtained by direct cell reprogramming approaches (Cat no:27867-1-AP)

FH

Joseph (Verified Customer) (01-14-2020)

I tested the Titin antibody using IHC on 16uM mouse skeletal muscle sections (Tibialis anterior and Soleus) using 1:100 and 1:300 dilutions. Both dilutions worked very well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse Skeletal Muscle - Soleus, tibialis anterior