Anticorps Monoclonal anti-TWIST2

TWIST2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1B12D11

N° de cat : 66544-1-Ig

Synonymes

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

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Galerie de données de validation

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  WB analysis using 66544-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66544-1-Ig (TWIST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human ovary tumor using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66544-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens).

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  IF Staining of HepG2 using 66544-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66544-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66544-1-Ig cible TWIST2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TWIST2 Protéine recombinante Ag2348
• Nom complet: twist homolog 2 (Drosophila)
• Masse moléculaire calculée: 160 aa, 18 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC033168
• Symbole du gène: TWIST2
• Identification du gène (NCBI): 117581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Publications

Species	Application	Title
human	WB	J Transl Med; VCP interaction with HMGB1 promotes hepatocellular carcinoma progression by activating the PI3K/AKT/mTOR pathway.; Authors - Zhangya Pu
human	IHC	J Healthc Eng; Expression of Dickkopf-1 and Twist2 in Cervical Squamous Cell Carcinoma and Their Correlation with Vasculogenic Mimicry.; Authors - Beibei Wang
mouse	IF	Nat Commun; Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration; Authors - Yang Yang