Anticorps Monoclonal anti-Prealbumin/transthyretin

Prealbumin/transthyretin Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1H8C4

N° de cat : 66108-1-Ig

Synonymes

ATTR, HsT2651, PALB, Prealbumin, Prealbumin/transthyretin, TBPA, transthyretin, TTR

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Galerie de données de validation

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  WB analysis of human brain using 66108-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66108-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66108-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50.

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  IHC staining of human liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50.

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  IHC staining of mouse liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66108-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of PC-12 using 66108-1-Ig

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Prealbumin/transthyretin (66108-1-Ig, Clone:1H8C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cerveau humain, tissu cardiaque humain
• Résultats positifs en IHC: tissu hépatique humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66108-1-Ig cible Prealbumin/transthyretin dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Prealbumin/transthyretin Protéine recombinante Ag19232
• Nom complet: transthyretin
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 14-16 kDa, 28-33 kDa
• Numéro d’acquisition GenBank: BC020791
• Symbole du gène: TTR
• Identification du gène (NCBI): 7276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)

Publications

Species	Application	Title
human	WB	Nat Commun; Secondary structure RNA elements control the cleavage activity of DICER.; Authors - Trung Duc Nguyen
mouse	WB	Am J Pathol; Renal function in aged C57BL/6J mice is impaired by deposition of age-related apolipoprotein A-II amyloid independent of kidney aging; Authors - Ying Li