Anticorps Monoclonal anti-TSPO/PBR

TSPO/PBR Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1D1F12

N° de cat : 68137-1-Ig

Synonymes

BZRP, DBI, IBP, MBR, mDRC, PBR, pk18, PKBS, PTBR, Translocator protein, translocator protein (18kDa), TSPO

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Galerie de données de validation

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  WB analysis using 68137-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68137-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of Jurkat using 68137-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 68137-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TSPO/PBR antibody (68137-1-Ig, Clone: 1D1F12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules 4T1, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IF: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68137-1-Ig cible TSPO/PBR dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TSPO/PBR Protéine recombinante Ag7475
• Nom complet: translocator protein (18kDa)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC001110
• Symbole du gène: TSPO
• Identification du gène (NCBI): 706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).