Anticorps Polyclonal de lapin anti-TSC22D4

• Phare
• Validé par KD/KO

TSC22D4 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 55017-1-AP

Synonymes

THG 1, THG1, TILZ2, Tsc 22 like protein THG 1, TSC22 domain family protein 4, TSC22 domain family, member 4, TSC22D4

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Galerie de données de validation

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  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 55017-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55017-1-AP (TSC22D4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

55017-1-AP cible TSC22D4 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TSC22 domain family, member 4
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41-45 kDa
• Numéro d’acquisition GenBank: NM_030935
• Symbole du gène: TSC22D4
• Identification du gène (NCBI): 81628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC22D4, also named as TILZ2 and THG-1, is a transcriptional repressor. It is a leucine zipper-containing protein that is highly conserved during evolution. TSC22D4 is transcriptionally up-regulated by many different stimuli, including anti-cancer drugs and growth inhibitors. It plays a suppressive role in tumorigenesis. TSC22D4 interacts with TSC22D1 by forming heterodimers(PMID:10488076).This antibody is specific to TSC22D4.

Publications

Species	Application	Title
human	WB,IP	J Cell Physiol; The interplay between TGF-β-stimulated TSC22 domain family proteins regulates cell-cycle dynamics in medulloblastoma cells; Authors - Jessica Dragotto ; KD Validated