Anticorps Polyclonal de lapin anti-TRIM63

• Phare
• Validé par KD/KO

TRIM63 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

65

Avis (1)

N° de cat : 55456-1-AP

Synonymes

IRF, Iris RING finger protein, MuRF 1, MURF1, MURF2, RING finger protein 28, RNF28, SMRZ, TRIM63, tripartite motif containing 63

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Galerie de données de validation

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  WB analysis of mouse heart using 55456-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55456-1-AP (TRIM63 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55456-1-AP (TRIM63 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55456-1-AP (TRIM63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55456-1-AP (TRIM63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 55456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55456-1-AP (TRIM63 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55456-1-AP

  Immunofluorescent analysis of HeLa cells using 55456-1-AP (TRIM63 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu cérébral de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 62 publications below
• IHC: See 4 publications below

Informations sur le produit

55456-1-AP cible TRIM63 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tripartite motif-containing 63
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_032588
• Symbole du gène: TRIM63
• Identification du gène (NCBI): 84676
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
mouse	WB	J Cachexia Sarcopenia Muscle; Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.; Authors - Guo-Kai Shang
mouse	WB	J Cachexia Sarcopenia Muscle; Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα+ mesenchymal progenitors.; Authors - Guo-Kai Shang
mouse	WB	J Cachexia Sarcopenia Muscle; Serum insulin-like growth factor binding protein 2 levels as biomarker for pancreatic ductal adenocarcinoma-associated malnutrition and muscle wasting.; Authors - Jie Dong
mouse	WB	Cell Rep; PMID: 29972780; Authors - Ravi K Singh
rat	WB	Redox Biol; Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.; Authors - Yuehong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brice-Emmanuel (Verified Customer) (10-12-2023)

I don't have the band at 40 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Heart Tissue

TRIM63 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:55456-1-AP)