Anticorps Polyclonal de lapin anti-TRIM26

TRIM26 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 27013-1-AP

Synonymes

Acid finger protein, AFP, RING finger protein 95, RNF95, TRIM26, tripartite motif containing 26, Zinc finger protein 173, ZNF173

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Galerie de données de validation

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  WB analysis using 27013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 27013-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 27013-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 27013-1-AP

  IP Result of anti-TRIM26 (IP:27013-1-AP, 4ug; Detection:27013-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules RAW 264.7, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

27013-1-AP cible TRIM26 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM26 Protéine recombinante Ag25546
• Nom complet: tripartite motif-containing 26
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC024039
• Symbole du gène: TRIM26
• Identification du gène (NCBI): 7726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma; Authors - Zhangjie Wang
pig	WB	Virus Res; TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection.; Authors - Pandeng Zhao
mouse	WB	Cell Death Dis; USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression; Authors - Weiwei Wang
human	WB,IHC,IF,IP	Int J Biol Sci; TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation; Authors - Yuening Sun ; KD Validated
human	WB,IF	FASEB J; TRIM26 restricts Epstein-Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β; Authors - Mingjiao Zhang ; KD Validated