Anticorps Monoclonal anti-TRIM24

TRIM24 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D6D9

N° de cat : 66324-1-Ig

Synonymes

hTIF1, PTC6, RING finger protein 82, RNF82, TF1A, TIF1, TIF1 alpha, TIF1A, TIF1ALPHA, TRIM24, tripartite motif containing 24

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Galerie de données de validation

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  WB analysis using 66324-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66324-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66324-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66324-1-Ig cible TRIM24 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRIM24 Protéine recombinante Ag5417
• Nom complet: tripartite motif-containing 24
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC028689
• Symbole du gène: TRIM24
• Identification du gène (NCBI): 8805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.