Anticorps Polyclonal de lapin anti-TREM2

TREM2 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 27599-1-AP

Synonymes

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

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Galerie de données de validation

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  WB analysis using 27599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27599-1-AP (TREM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of BV-2 using 27599-1-AP

  1X10^6 BV-2 cells were intracellularly stained with 0.4 ug Anti-Human TREM2 (27599-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MKN-45, cellules THP-1
• Résultats positifs en cytométrie: BV-2 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

27599-1-AP cible TREM2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TREM2 Protéine recombinante Ag26319
• Nom complet: triggering receptor expressed on myeloid cells 2
• Masse moléculaire calculée: 222 aa, 25 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC032362
• Symbole du gène: TREM2
• Identification du gène (NCBI): 54209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

Publications

Species	Application	Title
human	IF	Cell Death Dis; Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study.; Authors - Wen-Jin Chen
human,mouse	WB	Sci Adv; TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma; Authors - Rui Sun
human	IF	Front Immunol; Identification of TREM2-positive tumor-associated macrophages in esophageal squamous cell carcinoma: implication for poor prognosis and immunotherapy modulation; Authors - Hongmu Li
human,mouse	WB,IHC,IF	Nat Commun; Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease; Authors - Xingyu Zhang
mouse	WB	Brain Res; TREM2 regulates BV2 microglia activation and influences corticosterone-induced neuroinflammation in depressive disorders; Authors - Jingjing Shi