Anticorps Monoclonal anti-TRAF5

TRAF5 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

1B1A7

N° de cat : 66991-1-Ig

Synonymes

RING finger protein 84, RNF84, TRAF5

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Galerie de données de validation

• 

  WB analysis of HeLa using 66991-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66991-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66991-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules LNCaP, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66991-1-Ig cible TRAF5 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRAF5 Protéine recombinante Ag26070
• Nom complet: TNF receptor-associated factor 5
• Masse moléculaire calculée: 557 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC029600
• Symbole du gène: TRAF5
• Identification du gène (NCBI): 7188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299). The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).