Anticorps Polyclonal de lapin anti-TRAF2

• Phare
• Validé par KD/KO

TRAF2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 26846-1-AP

Synonymes

TRAF2, TRAP, TRAP3

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Galerie de données de validation

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  WB analysis of HepG2 using 26846-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26846-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 26846-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 26846-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRAF2 antibody (26846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 26846-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 26846-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 26846-1-AP (TRAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549, cellules HeLa, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

26846-1-AP cible TRAF2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAF2 Protéine recombinante Ag25387
• Nom complet: TNF receptor-associated factor 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC032410
• Symbole du gène: TRAF2
• Identification du gène (NCBI): 7186
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
mouse	WB	J Hazard Mater; Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.; Authors - Qiushuang Zhu
human	WB	EMBO Rep; Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1.; Authors - Hao Jia ; KD Validated
human,mouse	WB,IHC,IF	Free Radic Biol Med; TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis; Authors - Jiping Yao ; KD Validated
mouse	IF	J Nanobiotechnology; The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation.; Authors - Yanglin Wu