Anticorps Monoclonal anti-TPD52L2

TPD52L2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3B3C6

N° de cat : 68077-1-Ig

Synonymes

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

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Galerie de données de validation

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  WB analysis using 68077-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 68077-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 68077-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 68077-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 68077-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 68077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 68077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HCT 116 using 68077-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using TPD52L2 antibody (68077-1-Ig, Clone: 3B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: Calu-3 cells, cellules C6, cellules HCT 116, cellules MCF-7, cellules NCI-H1299, cellules Neuro-2a
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68077-1-Ig cible TPD52L2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor protein D52-like 2
• Masse moléculaire calculée: 206 aa, 22 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC006804
• Symbole du gène: TPD52L2
• Identification du gène (NCBI): 7165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.