Anticorps Monoclonal anti-TP73

TP73 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1D12A12

N° de cat : 66990-1-Ig

Synonymes

p53 like transcription factor, p53 related protein, P73, TP73, tumor protein p73

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Galerie de données de validation

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  WB analysis of MCF-7 using 66990-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66990-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66990-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66990-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66990-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66990-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66990-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66990-1-Ig (TP73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

66990-1-Ig cible TP73 dans les applications de WB, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TP73 Protéine recombinante Ag26793
• Nom complet: tumor protein p73
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_001126240
• Symbole du gène: TP73
• Identification du gène (NCBI): 7161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	RIP	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang
human	WB	Biochem Pharmacol; Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.; Authors - Xiaojiang Liu
human	WB	Carcinogenesis; Construction and identification of lncRNA/circRNA coregulated ceRNA networks in gemcitabine-resistant bladder carcinoma; Authors - Jingjing Pan