Anticorps Monoclonal anti-TNF Alpha

TNF Alpha Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11H8A5

N° de cat : CL488-69002

Synonymes

Cachectin, DIF, NeutraKine® TNF Alpha, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, Tumor necrosis factor

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Galerie de données de validation

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  FC experiment of human PBMCs using CL488-69002

  1X10^6 PMA+ionomycin-stimulated and Monensin Solution treated human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using CL488-69002

  1X10^6 human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of THP-1 using CL488-69002

  1X10^6 PMA, LPS, and Brefeldin A treated THP-1 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5) (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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  FC experiment of NK92 using CL488-69002

  1X10^6 PMA, ionomycin, and Brefeldin ANK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TNF Alpha (CL488-69002, Clone:11H8A5) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines stimulées par PMA + ionomycine en solution de monensine, cellules THP-1 traitées au PMA

Dilution recommandée

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-69002 cible TNF Alpha dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNF Alpha Protéine recombinante HZ-1014
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Symbole du gène: TNF
• Identification du gène (NCBI): 7124
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C