- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-TMEM119
TMEM119 Monoclonal Antibody for WB, ELISA
Hôte / Isotype
Mouse / IgG3
Réactivité testée
Humain, Lapin, porc, rat, souris
Applications
WB, FC, ELISA
Conjugaison
Non conjugué
CloneNo.
1E7A2
N° de cat : 66948-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de poulet, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de lapin, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| FC | See 1 publications below |
Informations sur le produit
66948-1-Ig cible TMEM119 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris
| Réactivité | Humain, Lapin, porc, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG3 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | TMEM119 Protéine recombinante Ag26286 |
| Nom complet | transmembrane protein 119 |
| Masse moléculaire calculée | 29 kDa |
| Poids moléculaire observé | 52 kDa |
| Numéro d’acquisition GenBank | NM_181724 |
| Symbole du gène | TMEM119 |
| Identification du gène (NCBI) | 338773 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#66948-1-Ig. The predicted MW of TMEM119 is 29 kDa. Catalog#66948-1-Ig recognizes 50 kDa band may due to glycosylation.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM119 antibody 66948-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia | ||
J Extracell Vesicles Characterization of brain-derived extracellular vesicles reveals changes in cellular origin after stroke and enrichment of the prion protein with a potential role in cellular uptake. | ||
Int J Biol Sci Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice | ||
Oxid Med Cell Longev Methane-Rich Saline Alleviates CA/CPR Brain Injury by Inhibiting Oxidative Stress, Microglial Activation-Induced Inflammatory Responses, and ER Stress-Mediated Apoptosis. | ||
Neural Regen Res Spi1 regulates the microglial/macrophage inflammatory response via the PI3K/AKT/mTOR signaling pathway after intracerebral hemorrhage | ||
J Neuroinflammation Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Amirah (Verified Customer) (03-02-2023) | The antibody works using the antigen retrieval method beforehand and using normal goat serum as a blocking buffer. Images not perfect but it still allows the antibody to be expressed.
|
FH Hannes (Verified Customer) (03-08-2021) | The antibody did not work in IHC-p on human and murine brain tissue.
 |
