Anticorps Monoclonal anti-TIMELESS

TIMELESS Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B6G3

N° de cat : 67022-1-Ig

Synonymes

FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

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Galerie de données de validation

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  WB analysis using 67022-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of A549 using 67022-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of JAR using 67022-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67022-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67022-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67022-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 67022-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 67022-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HSC-T6, cellules JAR, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules U2OS
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

67022-1-Ig cible TIMELESS dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIMELESS Protéine recombinante Ag6283
• Nom complet: timeless homolog (Drosophila)
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC050557
• Symbole du gène: TIMELESS
• Identification du gène (NCBI): 8914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Publications

Species	Application	Title
human,canine	WB	Eur J Pharmacol; TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation; Authors - Houqing Yin ; KD Validated