Anticorps Monoclonal anti-TIAR

TIAR Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1E8D12

N° de cat : 66907-1-Ig

Synonymes

Nucleolysin TIAR, TCBP, TIA 1 related protein, TIAL1, TIAR

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Galerie de données de validation

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  WB analysis using 66907-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66907-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66907-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66907-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66907-1-Ig (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66907-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66907-1-Ig (TIAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66907-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66907-1-Ig (TIAR antibody) at dilution of 1:100.

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  FC experiment of MCF-7 using 66907-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human TIAR (66907-1-Ig, Clone:1E8D12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules RAW 264.7, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66907-1-Ig cible TIAR dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIAR Protéine recombinante Ag11981
• Nom complet: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 39 and 41 kDa
• Numéro d’acquisition GenBank: BC030025
• Symbole du gène: TIAL1
• Identification du gène (NCBI): 7073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites. 66907-1-Ig detects 39 and 41 kDa bands in SDS-PAGE. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

Publications

Species	Application	Title
human	WB	Cell Biosci; Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.; Authors - Yuzo Abe
human	WB	J Cell Mol Med; LOXL1-AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR-374b-5p/MMP14 axis.; Authors - Bolong Yi
human	IF	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang
human	IF	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang