Anticorps Monoclonal anti-TGIF2

TGIF2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2D6B6

N° de cat : 67576-1-Ig

Synonymes

5 TG 3 interacting factor 2, Homeobox protein TGIF2, TGFB induced factor 2, TGFB induced factor homeobox 2, TGIF2

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Galerie de données de validation

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  WB analysis using 67576-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67576-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 67576-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules Daudi, cellules HL-60, cellules Jurkat, cellules K-562, cellules MOLT-4, cellules Ramos, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67576-1-Ig cible TGIF2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TGIF2 Protéine recombinante Ag30083
• Nom complet: TGFB-induced factor homeobox 2
• Masse moléculaire calculée: 237 aa, 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012816
• Symbole du gène: TGIF2
• Identification du gène (NCBI): 60436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGIF2, is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein.

Publications

Species	Application	Title
human	WB	Bosn J Basic Med Sci; Circ-RNF13, as an oncogene, regulates malignant progression of HBV-associated hepatocellular carcinoma cells and HBV infection through ceRNA pathway of circ-RNF13/miR-424-5p/TGIF2.; Authors - Yan Chen