Anticorps Monoclonal anti-TGFBI / BIGH3

• Phare
• Validé par KD/KO

TGFBI / BIGH3 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3E11D11

N° de cat : CL594-60007

Synonymes

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

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Galerie de données de validation

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  IF Staining of human colon cancer using CL594-60007

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-60007

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

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  FC experiment of Y79 using CL594-60007

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TGFBI / BIGH3 (CL594-60007, Clone:3E11D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu de cancer du côlon humain,
• Résultats positifs en cytométrie: cellules Y79

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60007 cible TGFBI / BIGH3 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TGFBI / BIGH3 Protéine recombinante Ag0241
• Nom complet: transforming growth factor, beta-induced, 68kDa
• Masse moléculaire calculée: 683 aa, 75 kDa
• Numéro d’acquisition GenBank: BC000097
• Symbole du gène: TGFBI
• Identification du gène (NCBI): 7045
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C