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Anticorps Monoclonal anti-TGFBI / BIGH3

TGFBI / BIGH3 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E11D11

N° de cat : CL488-60007

Synonymes

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated TGFBI / BIGH3 Monocl (CL488-60007)

    IF Staining of human colon cancer using CL488-60007

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 TGFBI / BIGH3 antibody (CL488-60007, Clone: 3E11D11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated TGFBI / BIGH3 Monocl (CL488-60007)

    IF Staining of human colon cancer using CL488-60007

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 TGFBI / BIGH3 antibody (CL488-60007, Clone: 3E11D11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Y79 cells using CoraLite® Plus 488-conjugated TGFBI / BIGH3 Monocl (CL488-60007)

    FC experiment of Y79 using CL488-60007

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TGFBI / BIGH3 (CL488-60007, Clone:3E11D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu de cancer du côlon humain,
Résultats positifs en cytométriecellules Y79

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60007 cible TGFBI / BIGH3 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène TGFBI / BIGH3 Protéine recombinante Ag0241
Nom complet transforming growth factor, beta-induced, 68kDa
Masse moléculaire calculée 683 aa, 75 kDa
Numéro d’acquisition GenBankBC000097
Symbole du gène TGFBI
Identification du gène (NCBI) 7045
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 TGFBI / BIGH3 antibody CL488-60007Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Parallelized multidimensional analytic framework applied to mammary epithelial cells uncovers regulatory principles in EMT

Authors - Indranil Paul
View Article