Anticorps Monoclonal anti-Thyroglobulin

Thyroglobulin Monoclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4D3C5

N° de cat : 60272-1-Ig

Synonymes

AITD3, TG, TGN, thyroglobulin

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Galerie de données de validation

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  IHC staining of human thyroid using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human thyroid cancer using 60272-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using Thyroglobulin antibody (60272-1-Ig, Clone: 4D3C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human thyroid cancer using 60272-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using Thyroglobulin antibody (60272-1-Ig, Clone: 4D3C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu thyroïdien humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer de la thyroïde humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60272-1-Ig cible Thyroglobulin dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Thyroglobulin Protéine recombinante Ag18070
• Nom complet: thyroglobulin
• Masse moléculaire calculée: 2768 aa, 305 kDa
• Numéro d’acquisition GenBank: BC140933
• Symbole du gène: TG
• Identification du gène (NCBI): 7038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.

Publications

Species	Application	Title
human	IF	Front Immunol; Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto's Thyroiditis.; Authors - Hui Xiao
human	IHC	Cancer Cell Int; Identification of an immune-related signature indicating the dedifferentiation of thyroid cells.; Authors - Xuemin Wang