Anticorps Monoclonal anti-TEX14

TEX14 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1A11D6

N° de cat : 67982-1-Ig

Synonymes

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

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Galerie de données de validation

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  WB analysis of rat testis using 67982-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 67982-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit testis using 67982-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 67982-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 67982-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67982-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de rat, cellules PC-3, tissu testiculaire de lapin, tissu testiculaire de souris
• Résultats positifs en IF: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67982-1-Ig cible TEX14 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TEX14 Protéine recombinante Ag13143
• Nom complet: testis expressed 14
• Masse moléculaire calculée: 957aa,107 kDa; 1497aa,168 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC040526
• Symbole du gène: TEX14
• Identification du gène (NCBI): 56155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.