Anticorps Monoclonal anti-TERF2

TERF2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

5B1E1

N° de cat : 66893-1-Ig

Synonymes

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

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Galerie de données de validation

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  WB analysis of MCF-7 using 66893-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66893-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66893-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66893-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66893-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66893-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66893-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66893-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66893-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66893-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66893-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66893-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules 4T1, cellules Daudi, cellules HEK-293, cellules HSC-T6, cellules HT-29, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66893-1-Ig cible TERF2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TERF2 Protéine recombinante Ag28513
• Nom complet: telomeric repeat binding factor 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 60-65.32-35 kDa
• Numéro d’acquisition GenBank: BC024890
• Symbole du gène: TERF2
• Identification du gène (NCBI): 7014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.

Publications

Species	Application	Title
human	IF	Sci Adv; Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).; Authors - Ji Hoon Lee
human,mouse	WB	Front Pharmacol; Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.; Authors - Xiaoyu Qi