Anticorps Monoclonal anti-TELO2

TELO2 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3H10F4

N° de cat : 66077-1-Ig

Synonymes

hCLK2, KIAA0683, Protein clk 2 homolog, TEL2, TELO2

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Galerie de données de validation

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  WB analysis using 66077-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 66077-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66077-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66077-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66077-1-Ig (TELO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 66077-1-Ig

  IP Result of anti-TELO2 (IP:66077-1-Ig, 4ug; Detection:66077-1-Ig 1:500) with HepG2 cells lysate 2900ug.

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  IHC staining of human kidney using 66077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66077-1-Ig (TELO2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 66077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 66077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66077-1-Ig(TELO2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66077-1-Ig

  Immunofluorescent analysis of () fixed HepG2 cells using 66077-1-Ig (TELO2 antibody) at dilution of 1:25.

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules A431, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules L02, cellules MCF-7
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66077-1-Ig cible TELO2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TELO2 Protéine recombinante Ag8834
• Nom complet: TEL2, telomere maintenance 2, homolog (S. cerevisiae)
• Masse moléculaire calculée: 837 aa, 92 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC017188
• Symbole du gène: TELO2
• Identification du gène (NCBI): 9894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), and ultraviolet (UV). The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.

Publications

Species	Application	Title
human	WB	Mol Cell; Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2.; Authors - Feng Rao