- Phare
- Validé par KD/KO
Anticorps Recombinant de lapin anti-TDP-43
TDP-43 Recombinant Antibody for FC (Intra), IF
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
IF, FC (Intra)
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
11N20
N° de cat : CL488-80001
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF | cellules HeLa, |
Résultats positifs en cytométrie | cellules HeLa |
Dilution recommandée
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-80001 cible TDP-43 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Recombinant |
Type | Anticorps |
Immunogène | TDP-43 Protéine recombinante Ag1231 |
Nom complet | TAR DNA binding protein |
Masse moléculaire calculée | 43 kDa |
Poids moléculaire observé | 43 kDa |
Numéro d’acquisition GenBank | BC001487 |
Symbole du gène | TARDBP |
Identification du gène (NCBI) | 23435 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.
In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).
80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.
Protocole
Product Specific Protocols | |
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IF protocol for CL Plus 488 TDP-43 antibody CL488-80001 | Download protocol |
FC protocol for CL Plus 488 TDP-43 antibody CL488-80001 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |