Anticorps Monoclonal anti-TBR1

TBR1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

5C3A5

N° de cat : 66564-1-Ig

Synonymes

T box brain protein 1, T box, brain, 1, T brain 1, TBR 1, TBR1, TES 56

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Galerie de données de validation

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  WB analysis using 66564-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of pig cerebellum using 66564-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of rat cerebellum using 66564-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IHC staining of mouse brain using 66564-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66564-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66564-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66564-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66564-1-Ig (TBR1 antibody) at dilution of 1:100 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 66564-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66564-1-Ig (TBR1 antibody) at dilution of 1:100 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

66564-1-Ig cible TBR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TBR1 Protéine recombinante Ag15262
• Nom complet: T-box, brain, 1
• Masse moléculaire calculée: 682 aa, 74 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC104844
• Symbole du gène: TBR1
• Identification du gène (NCBI): 10716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.

Publications

Species	Application	Title
mouse	IF	Nat Commun; LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.; Authors - Huai-Bin Hu
mouse	IHC	EMBO J; Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway.; Authors - Thao P Phan
mouse	IF	Ann Neurol; Pathogenic MAST3 Variants in the STK Domain Are Associated with Epilepsy.; Authors - Egidio Spinelli
mouse	IF	Biochem Biophys Res Commun; Neuron-specific loss of Ppp6c induces neonatal death and decreases the number of cortical neurons and interneurons; Authors - Miki Matsuoka
human	IHC	PLoS One; Cortical spheroid on perfusable microvascular network in a microfluidic device; Authors - Teal Russell