Anticorps Monoclonal anti-WWTR1

• Phare
• Validé par KD/KO

WWTR1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

2A12A10

N° de cat : 66500-1-Ig

Synonymes

TAZ, WWTR1

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Galerie de données de validation

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  WB analysis using 66500-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66500-1-Ig

  WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells.

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  WB analysis using 66500-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66500-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66500-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 66500-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231, cellules MDA-MB-453s, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du foie humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

66500-1-Ig cible WWTR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WWTR1 Protéine recombinante Ag13330
• Nom complet: WW domain containing transcription regulator 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC014052
• Symbole du gène: WWTR1
• Identification du gène (NCBI): 25937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma; Authors - Yu-Fei Cao
human	WB	Nat Commun; LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.; Authors - Huai-Bin Hu ; KD Validated
mouse	WB,IHC,IF	Front Pharmacol; TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury.; Authors - Xuyang Hu
human	WB	Am J Transl Res; VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.; Authors - Adheesh Bhandari
rat	WB,IF	Toxicol Appl Pharmacol; Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif; Authors - Xinghe Chen
human	WB,IHC	Stem Cells Dev; Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.; Authors - Shaojun Nong