Anticorps Monoclonal anti-TAU

• Phare
• Validé par KD/KO

TAU Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2c

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

1E9A8

Avis (2)

N° de cat : 66499-1-Ig

Synonymes

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

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Galerie de données de validation

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  WB analysis of HeLa using 66499-1-Ig

  WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells.

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  WB analysis using 66499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Neuro-2a, cellules SH-SY5Y, cellules U-251, cellules Y79, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:30000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

66499-1-Ig cible TAU dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2c
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAU Protéine recombinante Ag21926
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: MAPT
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.

Publications

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.; Authors - Xue Wang
human	WB	Int J Mol Sci; Neuroprotection and Mechanism of Gas-miR36-5p from Gastrodia elata in an Alzheimer's Disease Model by Regulating Glycogen Synthase Kinase-3β; Authors - Zhongteng Lu
mouse	WB	Front Aging Neurosci; Salidroside Ameliorates Alzheimer's Disease by Targeting NLRP3 Inflammasome-Mediated Pyroptosis.; Authors - Yawen Cai
mouse	WB	Mol Med; Eriodictyol ameliorates cognitive dysfunction in APP/PS1 mice by inhibiting ferroptosis via vitamin D receptor-mediated Nrf2 activation.; Authors - Lin Li
mouse	WB	Environ Sci Pollut Res Int; Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells.; Authors - Lingli Chen
mouse	WB	Comput Math Methods Med; Acupuncture Effect Assessment in APP/PS1 Transgenic Mice: On Regulating Learning-Memory Abilities, Gut Microbiota, and Microbial Metabolites.; Authors - Bo Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Virender (Verified Customer) (01-09-2020)

Tau antibody works well for recombinant tau as well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000

FH

Mary (Verified Customer) (11-08-2019)

I like this antibody. It provides a clear specific signal. The shipping was prompt and the antibody was kept on ice the whole time which I appreciated.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y