Anticorps Monoclonal anti-TAOK3

TAOK3 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1A5A9

N° de cat : 67451-1-Ig

Synonymes

DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

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Galerie de données de validation

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  WB analysis using 67451-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67451-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67451-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67451-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67451-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 67451-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67451-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67451-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human prostate cancer using 67451-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human prostate cancer using 67451-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer de la prostate humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67451-1-Ig cible TAOK3 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAOK3 Protéine recombinante Ag29146
• Nom complet: TAO kinase 3
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC002756
• Symbole du gène: TAOK3
• Identification du gène (NCBI): 51347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

Publications

Species	Application	Title
human	WB,IHC,CoIP	Adv Sci (Weinh); TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM; Authors - Mingchuang Sun ; KD Validated