Anticorps Polyclonal de lapin anti-TAB1

• Phare
• Validé par KD/KO

TAB1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 27566-1-AP

Synonymes

3' Tab1, MAP3K7IP1, TAB1, TAK1 binding protein 1

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Galerie de données de validation

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  WB analysis using 27566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 27566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27566-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27566-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27566-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 27566-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules A375, cellules HeLa, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

27566-1-AP cible TAB1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAB1 Protéine recombinante Ag25576
• Nom complet: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC050554
• Symbole du gène: MAP3K7IP1
• Identification du gène (NCBI): 10454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Theranostics; Virus-induced p38 MAPK activation facilitates viral infection.; Authors - Yuting Cheng ; KO Validated