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Anticorps Monoclonal anti-Synaptophysin

Synaptophysin Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4E12C4

N° de cat : CL594-67864

Synonymes

synaptophysin, SYP

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:2000.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    IF Staining of mouse brain using CL594-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Synaptophysin antibody (CL594-67864, Clone: 4E12C4 ) at dilution of 1:2000.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite®594-conjugated Synaptophysin Monoclonal a (CL594-67864)

    FC experiment of PC-12 using CL594-67864

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Synaptophysin (CL594-67864, Clone:4E12C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu cérébral de souris,
Résultats positifs en cytométriecellules PC-12

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67864 cible Synaptophysin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Synaptophysin Protéine recombinante Ag11803
Nom complet synaptophysin
Masse moléculaire calculée 313 aa, 34 kDa
Numéro d’acquisition GenBankBC064550
Symbole du gène SYP
Identification du gène (NCBI) 6855
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocole

Product Specific Protocols
IF protocol for CL594 Synaptophysin antibody CL594-67864Download protocol
FC protocol for CL594 Synaptophysin antibody CL594-67864Download protocol
Standard Protocols
Click here to view our Standard Protocols