• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anticorps Monoclonal anti-Stanniocalcin 2

Stanniocalcin 2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4H1E7

N° de cat : CL594-60063

Synonymes

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Stanniocalcin 2 Monoclonal (CL594-60063)

    IF Staining of HeLa using CL594-60063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60063 (Stanniocalcin 2 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Stanniocalcin 2 Monoclonal (CL594-60063)

    FC experiment of HeLa using CL594-60063

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Stanniocalcin 2 (CL594-60063, Clone:4H1E7) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60063 cible Stanniocalcin 2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Stanniocalcin 2 Protéine recombinante Ag0359
Nom complet stanniocalcin 2
Masse moléculaire calculée 33 kDa
Poids moléculaire observé 33 kDa
Numéro d’acquisition GenBankBC000658
Symbole du gène STC2
Identification du gène (NCBI) 8614
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Protocole

Product Specific Protocols
IF protocol for CL594 Stanniocalcin 2 antibody CL594-60063Download protocol
Standard Protocols
Click here to view our Standard Protocols