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Anticorps Polyclonal de lapin anti-Siglec-15

Siglec-15 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 28594-1-AP

Synonymes

CD33 antigen like 3, CD33L3, HsT1361, SIGLEC 15, SIGLEC15

Galerie de données de validation

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  • Western Blot (WB) analysis of A549 cells using Siglec-15 Polyclonal antibody (28594-1-AP)

    WB analysis of A549 using 28594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28594-1-AP (Siglec-15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules A549, cellules NCI-H1299, cellules PC-3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

28594-1-AP cible Siglec-15 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Siglec-15 Protéine recombinante Ag29829
Nom complet sialic acid binding Ig-like lectin 15
Masse moléculaire calculée 36 kDa
Poids moléculaire observé38-40 kDa
Numéro d’acquisition GenBankNM_213602
Symbole du gène SIGLEC15
Identification du gène (NCBI) 284266
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid binding Ig-like lectin 15 (Siglec-15) is a member of the Siglec family of glycan-recognition proteins. Siglec-15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail (PMID: 17483134). It is mainly expressed on a subset of myeloid lineage cells and can be upregulated in many cancers (PMID: 32939323). Siglec-15 has been reported as a immune suppressor and a potential target for normalization cancer immunotherapy (PMID: 30833750). Siglec-15 has a calculated molecular weight of 36 kDa. It has been detected at 38-40 kDa due to glycosylation (PMID: 32921411).

Protocole

Product Specific Protocols
WB protocol for Siglec-15 antibody 28594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Rebecca (Verified Customer) (07-21-2022)

Positive staining identified at 1:500 dilution in patient FFPE tissue. Slight background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer FFPE tissue
Siglec-15 Antibody Immunofluorescence validation (1:500 dilution) in Prostate cancer FFPE tissue (Cat no:28594-1-AP)