Anticorps Monoclonal anti-Sestrin 2

• Phare
• Validé par KD/KO

Sestrin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

CloneNo.

1A1A4

Avis (1)

N° de cat : 66297-1-Ig

Synonymes

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

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Galerie de données de validation

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  WB analysis of HEK-293T using 66297-1-Ig

  WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells.

• 

  WB analysis of HeLa using 66297-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66297-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66297-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66297-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66297-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66297-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66297-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66297-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, HEK-293T
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

66297-1-Ig cible Sestrin 2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Sestrin 2 Protéine recombinante Ag20638
• Nom complet: sestrin 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC013304
• Symbole du gène: SESN2
• Identification du gène (NCBI): 83667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Publications

Species	Application	Title
human,mouse	WB,IF	Cell Biol Toxicol; Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.; Authors - Yiming Jiang ; KD Validated
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
pig	WB, IP	J Nutr; Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.; Authors - Agus Suryawan
human,mouse	WB,IHC,IF,IP	Cell Tissue Res; Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy.; Authors - Yawei Bian
pig	WB	Am J Physiol Endocrinol Metab; Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs.; Authors - Marko Rudar
mouse	WB	Arch Pharm Res; Sestrin2 induction contributes to anti-inflammatory responses and cell survival by globular adiponectin in macrophages; Authors - Sumin Lee ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yahir Alberto (Verified Customer) (11-12-2021)

This antibody works nicely for western blotting, although with several unspecific bands for a monoclonal antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T cells

Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:66297-1-Ig)