Anticorps Monoclonal anti-SUMO2/3

• Phare
• Validé par KD/KO

SUMO2/3 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1A1B3

N° de cat : CL594-67154

Synonymes

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, Ubiquitin like protein SMT3B

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Galerie de données de validation

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  IF Staining of HeLa using CL594-67154

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-67154 (SUMO2/3 antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL594-67154

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SUMO2/3 antibody (CL594-67154, Clone: 1A1B3 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67154

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using CL594-67154

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67154 cible SUMO2/3 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SUMO2/3 Protéine recombinante Ag28672
• Nom complet: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC008450
• Symbole du gène: SUMO2
• Identification du gène (NCBI): 6613
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011