Anticorps Monoclonal anti-SUMO2/3

• Phare
• Validé par KD/KO

SUMO2/3 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1A1B3

N° de cat : 67154-1-Ig

Synonymes

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, Ubiquitin like protein SMT3B

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Galerie de données de validation

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  WB analysis using 67154-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67154-1-Ig

  WB result of SUMO2/3 antibody (67154-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells.

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  WB analysis of A549 using 67154-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67154-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67154-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67154-1-Ig cible SUMO2/3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SUMO2/3 Protéine recombinante Ag28672
• Nom complet: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC008450
• Symbole du gène: SUMO2
• Identification du gène (NCBI): 6613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila