Anticorps Polyclonal de lapin anti-STK25

• Phare
• Validé par KD/KO

STK25 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 25821-1-AP

Synonymes

SOK 1, SOK1, Ste20 like kinase, STK25, YSK1

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Galerie de données de validation

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  WB analysis using 25821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25821-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25821-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25821-1-AP

  IP Result of anti-STK25 (IP:25821-1-AP, 4ug; Detection:25821-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

25821-1-AP cible STK25 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STK25 Protéine recombinante Ag22964
• Nom complet: serine/threonine kinase 25 (STE20 homolog, yeast)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC015793
• Symbole du gène: STK25
• Identification du gène (NCBI): 10494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IP	Cell Mol Gastroenterol Hepatol; Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease.; Authors - Emmelie Cansby
human,mouse	WB	Cell Mol Gastroenterol Hepatol; Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways.; Authors - Yeshwant Kurhe ; KD Validated
human	WB	J Exp Clin Cancer Res; STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.; Authors - Fan Wu ; KD Validated
human	WB	Mol Carcinog; Downregulation of STK25 promotes autophagy via the Janus kinase 2/signal transducer and activator of transcription 3 pathway in colorectal cancer.; Authors - Jiangbo Chen
rat	WB	Cell Mol Neurobiol; Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways.; Authors - Zeli Song
mouse	WB	Biochem Biophys Res Commun; Loss of serine/threonine protein kinase 25 in retinal ganglion cells ameliorates high glucose-elicited damage through regulation of the AKT-GSK-3β/Nrf2 pathway.; Authors - Zhuolin Zhou ; KD Validated