Anticorps Monoclonal anti-STK11/LKB1

STK11/LKB1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D11E5

N° de cat : 68016-1-Ig

Synonymes

LKB1, PJS, serine/threonine kinase 11, STK11

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Galerie de données de validation

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  WB analysis of HEK-293 using 68016-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 68016-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 68016-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68016-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 68016-1-Ig

  Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 68016-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 68016-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HSC-T6 traitées à la calyculine A, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:9800
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68016-1-Ig cible STK11/LKB1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007981
• Symbole du gène: STK11
• Identification du gène (NCBI): 6794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.