Anticorps Polyclonal de lapin anti-STK10

STK10 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 25471-1-AP

Synonymes

LOK, Lymphocyte oriented kinase, PRO2729, serine/threonine kinase 10, STK10

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Galerie de données de validation

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  WB analysis of SKOV-3 using 25471-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25471-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25471-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25471-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 25471-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25471-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules A549, cellules COLO 320
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25471-1-AP cible STK10 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STK10 Protéine recombinante Ag22169
• Nom complet: serine/threonine kinase 10
• Masse moléculaire calculée: 968 aa, 112 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC070077
• Symbole du gène: STK10
• Identification du gène (NCBI): 6793
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressedas a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

Publications

Species	Application	Title
		Clin Cancer Res; Combined CDK4/6 and ERK1/2 inhibition enhances anti-tumor activity in NF1-associated plexiform neurofibroma; Authors - Alyssa C Flint

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jared (Verified Customer) (08-21-2019)

This antibody is an all-star antibody. I can even re use it a couple of times, and it still works. potent stuff. Picture would not upload... There is one strong band, and it shows virtually nothing in the lane where I CRISPR'd out LOK aka STK10 in the LOK band area. There is a non-specific band, but that is located clearly out of the range of where the LOK band is located. Non-specific band at approx 200kd. LOK for me runs at around 140kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: 293T and H1299

STK10 Antibody Western Blot, validation (1:3000 dilution) in 293T and H1299 (Cat no:25471-1-AP)