Anticorps Monoclonal anti-STAT5B

STAT5B Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1D9B11

N° de cat : CL594-66427

Synonymes

STAT5, STAT5B

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-66427

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 STAT5B antibody (CL594-66427, Clone: 1D9B11 ) at dilution of 1:200, CL488-Phalloidin (green).

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  FC experiment of HepG2 using CL594-66427

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human STAT5B (CL594-66427, Clone:1D9B11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66427 cible STAT5B dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STAT5B Protéine recombinante Ag2709
• Nom complet: signal transducer and activator of transcription 5B
• Masse moléculaire calculée: 787 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC020868
• Symbole du gène: STAT5B
• Identification du gène (NCBI): 6777
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).