Anticorps Polyclonal de lapin anti-STAT3

STAT3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 51076-2-AP

Synonymes

Acute phase response factor, APRF, HIES, STAT3

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Galerie de données de validation

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  WB analysis of K-562 using 51076-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 51076-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51076-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51076-2-AP

  IP Result of anti-STAT3 (IP:51076-2-AP, 4ug; Detection:51076-2-AP 1:500) with HeLa cells lysate 1500ug.

• 

  IHC staining of human cervical cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51076-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51076-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51076-2-AP (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51076-2-AP (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

51076-2-AP cible STAT3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 3 (acute-phase response factor)
• Masse moléculaire calculée: 770 aa, 88 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC000627
• Symbole du gène: STAT3
• Identification du gène (NCBI): 6774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publications

Species	Application	Title
mouse	WB	FASEB J; Lmo4-resistin signaling contributes to adipose tissue-liver crosstalk upon weight cycling.; Authors - Yu Sun
mouse	WB	Neurotox Res; Hispidulin Inhibits Neuroinflammation in Lipopolysaccharide-Activated BV2 Microglia and Attenuates the Activation of Akt, NF-κB, and STAT3 Pathway.; Authors - Chung-I Yu
human	WB	Oncol Lett; ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway.; Authors - Shengjin Yu
human	IP	Cell Biol Int; Identification and analysis of glycogen synthase kinase 3 beta1 interactome.; Authors - Gao Xuejuan X
		Mol Med Rep; A small GTPase?like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in?vitro via interaction with Rac1 and Stat3.; Authors - Hu Xiaopeng X