Anticorps Polyclonal de lapin anti-STAT2

STAT2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 51075-2-AP

Synonymes

ISGF 3, P113, STAT113, STAT2

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Galerie de données de validation

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  WB analysis of multi-cells using 51075-2-AP

  WB result of anti-STAT2 (51075-2-AP) in different cell lysates.

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  WB analysis of MCF-7 using 51075-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 51075-2-AP (STAT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51075-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51075-2-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF7, multi-cellules
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

51075-2-AP cible STAT2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 2, 113kDa
• Masse moléculaire calculée: 851 aa, 98 kDa
• Poids moléculaire observé: 100-113 kDa
• Numéro d’acquisition GenBank: BC051284
• Symbole du gène: STAT2
• Identification du gène (NCBI): 6773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publications

Species	Application	Title
human		J Clin Invest; Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy.; Authors - Yan-Ping Xu
human	WB	BMC Vet Res; Curcumol inhibits EMCV replication by activating CH25H and inhibiting the formation of ROs; Authors - Jiangang Zheng
human	WB	J Transl Med; Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils; Authors - Ye Xie
mouse	WB	Viruses; Interferon-Stimulated Gene 15 Knockout in Mice Impairs IFNα-Mediated Antiviral Activity; Authors - Chen Li