Anticorps Monoclonal anti-STAT1

• Phare
• Validé par KD/KO

STAT1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

CloneNo.

1F7C6

N° de cat : 66545-1-Ig

Synonymes

ISGF 3, STAT1, STAT91

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Galerie de données de validation

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  WB analysis using 66545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 66545-1-Ig

  WB result of STAT1 antibody (66545-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

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  WB analysis using 66545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66545-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66545-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 66545-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STAT1 antibody (66545-1-Ig, Clone: 1F7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66545-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (66545-1-Ig, Clone: 1F7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: IFN alpha treated HeLa cells, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66545-1-Ig cible STAT1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: STAT1 Protéine recombinante Ag0199
• Nom complet: signal transducer and activator of transcription 1, 91kDa
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC002704
• Symbole du gène: STAT1
• Identification du gène (NCBI): 6772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.

Publications

Species	Application	Title
mouse	WB,IF	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang
human	WB,IF	Int Immunopharmacol; Silencing SIRT1 promotes the anti-HBV action of IFN-α by regulating Pol expression and activating the JAK-STAT signaling pathway; Authors - Qinyan Tang
human	WB,IF	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du ; KD Validated
human	WB	PLoS Negl Trop Dis; Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response; Authors - Teng LiI
mouse	WB	FEBS J; Statins attenuate antiviral IFN-β and ISG expression via inhibition of IRF3 and JAK/STAT signaling in poly(I:C)-treated hyperlipidemic mice and macrophages.; Authors - Atsushi Koike
human	WB,IHC,IF	Chin J Nat Med; Panax notoginseng saponins prevent colitis-associated colorectal cancer via inhibition IDO1 mediated immune regulation.; Authors - Xue-Ming Li