Anticorps Monoclonal anti-SRPX2

• Phare
• Validé par KD/KO

SRPX2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2A1D7

N° de cat : 66266-1-Ig

Synonymes

BPP, CBPS, PMGX, RESDX, SRPUL, SRPX2

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Galerie de données de validation

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  WB analysis of pig lung using 66266-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66266-1-Ig (SRPX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66266-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66266-1-Ig (SRPX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66266-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66266-1-Ig (SRPX2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66266-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66266-1-Ig (SRPX2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 66266-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SRPX2 antibody (66266-1-Ig, Clone: 2A1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de porc, cellules A549
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66266-1-Ig cible SRPX2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SRPX2 Protéine recombinante Ag8084
• Nom complet: sushi-repeat-containing protein, X-linked 2
• Masse moléculaire calculée: 465 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC020733
• Symbole du gène: SRPX2
• Identification du gène (NCBI): 27286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRPX2 (sushi-repeat-containing protein, X-linked 2) is a secreted protein expressed in neurons of the human adult brain, including the rolandic area (PMID: 20874700). Firstly described as sushi-repeat protein up-regulated in leukemia (SPRUL) in leukemia cells with dysregulated expression at the transcriptional level, SRPX2 has been recently shown as a multifunctional protein. SRPX2 is a ligand for uPAR, the urokinase-type plasminogen activator (uPA) receptor (PMID: 18718938). It is involved in seizure disorders, angiogenesis and cellular adhesion (PMID: 22242148; 19667118; 16497722). The involvement of SRPX2 in disorders of language cortex suggests it has an important role in the perisylvian region critical for language and cognitive development (PMID: 16497722).

Publications

Species	Application	Title
mouse,human	WB,IHC,IF	J Comp Neurol; Sushi repeat-containing protein X-linked 2: A novel phylogenetically conserved hypothalamo-pituitary protein.; Authors - Mehwish Anwer ; KD Validated
human	WB,IHC	Onco Targets Ther; High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer.; Authors - Meng Zhang
human	WB	Aging (Albany NY); TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs); Authors - Jianyun Liu