Anticorps Monoclonal anti-SORD

SORD Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4A8E1

N° de cat : 67625-1-Ig

Synonymes

L iditol 2 dehydrogenase, SDH, sorbitol dehydrogenase, SORD, SORD1

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Galerie de données de validation

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  WB analysis using 67625-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HSC-T6 using 67625-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67625-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67625-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below

Informations sur le produit

67625-1-Ig cible SORD dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SORD Protéine recombinante Ag8651
• Nom complet: sorbitol dehydrogenase
• Masse moléculaire calculée: 357 aa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC021085
• Symbole du gène: SORD
• Identification du gène (NCBI): 6652
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

Publications

Species	Application	Title
mouse	WB	J Nutr; Sucrose intake elevates erythritol in plasma and urine in male mice; Authors - Semira R Ortiz ; KO Validated
human	WB	Front Nutr; Erythritol synthesis is elevated in response to oxidative stress and regulated by the non-oxidative pentose phosphate pathway in A549 cells; Authors - Semira R. Ortiz ; KD Validated