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Anticorps Monoclonal anti-SOD2

SOD2 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

3A6C2

N° de cat : CL555-66474

Synonymes

IPO B, Mn SOD, MNSOD, SOD2, SODM

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated SOD2 Monoclonal antibody (CL555-66474)

    FC experiment of HeLa using CL555-66474

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SOD2 (CL555-66474, Clone:3A6C2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-66474 cible SOD2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène SOD2 Protéine recombinante Ag21388
Nom complet superoxide dismutase 2, mitochondrial
Masse moléculaire calculée 25 kDa
Poids moléculaire observé 25 kDa
Numéro d’acquisition GenBankBC016934
Symbole du gène SOD2
Identification du gène (NCBI) 6648
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protocole

Product Specific Protocols
FC protocol for CL555 SOD2 antibody CL555-66474Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Anja (Verified Customer) (07-25-2022)

The antibody worked well for Flow Cytometry at a 1:100 dilution for isolated mouse cells. I would buy it again.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Thymic Epithelial Cells