Anticorps Monoclonal anti-SNX27

SNX27 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2C4G5

N° de cat : 68386-1-Ig

Synonymes

KIAA0488, MRT1, MY014, SNX27, Sorting nexin 27, sorting nexin family member 27

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Galerie de données de validation

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  WB analysis using 68386-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68386-1-Ig (SNX27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 68386-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNX27 antibody (68386-1-Ig, Clone: 2C4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 68386-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SNX27 antibody (68386-1-Ig, Clone: 2C4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP
• Résultats positifs en IF: cellules HeLa, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68386-1-Ig cible SNX27 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNX27 Protéine recombinante Ag9447
• Nom complet: sorting nexin family member 27
• Masse moléculaire calculée: 61 kDa, 60 kDa, 52 kDa, 28 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC012184
• Symbole du gène: SNX27
• Identification du gène (NCBI): 81609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sorting nexin 27 (SNX27), a PDZ domain-containing protein, belongs to the sorting nexin family of proteins. SNX27 promotes the recycling of internalized transmembrane proteins from endosomes to the plasma membrane by linking PDZ-dependent cargo recognition to retromer-mediated transport (PMID: 21602791; 23563491). It has been shown that SNX27 deficiency contributes to synaptic and cognitive deficits by modulating glutamate receptor recycling in Down's syndrome (PMID: 23524343). SNX27 has also been proposed to have a role in regulating β-amyloid (Aβ) generation by modulating γ-secretase activity, which is a molecular mechanism for Aβ-dependent pathogenesis in both Down's syndrome and Down's syndrome (PMID: 25437557).