Anticorps Monoclonal anti-SNAP25

SNAP25 Monoclonal Antibody for IF, WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3E4B7

N° de cat : CL594-60159

Synonymes

bA416N4.2, dJ1068F16.2, FLJ23079, RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

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Galerie de données de validation

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  WB analysis of mouse brain using CL594-60159

  mouse brain tissue were subjected to SDS PAGE followed by western blot with CL594-60159 (SNAP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using CL594-60159

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CL594-60159 (SNAP25 antibody) at dilution of 1:100.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IF: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60159 cible SNAP25 dans les applications de WB, IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNAP25 Protéine recombinante Ag6695
• Nom complet: synaptosomal-associated protein, 25kDa
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC010647
• Symbole du gène: SNAP25
• Identification du gène (NCBI): 6616
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.