Anticorps Monoclonal anti-SMO

SMO Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

CloneNo.

2B10A11

Avis (2)

N° de cat : 66851-1-Ig

Synonymes

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Galerie de données de validation

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  WB analysis using 66851-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66851-1-Ig (SMO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66851-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66851-1-Ig (SMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66851-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66851-1-Ig (SMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 66851-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66851-1-Ig (SMO antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66851-1-Ig cible SMO dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMO Protéine recombinante Ag25861
• Nom complet: smoothened homolog (Drosophila)
• Masse moléculaire calculée: 787 aa, 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC009989
• Symbole du gène: SMO
• Identification du gène (NCBI): 6608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Publications

Species	Application	Title
human	WB,IHC,IF	Int J Biol Sci; Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma; Authors - Wenjiao Jin
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Front Cell Dev Biol; Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.; Authors - Jiawei Li
rat	WB	Oxid Med Cell Longev; Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats; Authors - Xiaoqiong Li
human	WB	Cancer Sci; Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition.; Authors - Kaibo Guo
human	WB	FASEB J; Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway; Authors - Junjie Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aidan (Verified Customer) (08-31-2021)

Smo Monoclonal antibody responded to S. purpuratus Smo protein in both IF and WB experiments. For IF, methanol fixed embryos treated with 1:100 dilution of the antibody in PBS overnight, followed by incubation in a 1:300 dilution of secondary antibody Alexa Goat anti-mouse 488 diluted 1:300 in PBS. Localization appears peri-nuclear at the 16 cell stage.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: IF: 1:100 WB: 1:2000
• Cell Tissue Type: Strongylocentrotus purpuratus (Purple Sea Urchin) Embryo

SMO Antibody Western Blot,Immunofluorescence validation (IF: 1:100 WB: 1:2000 dilution) in Strongylocentrotus purpuratus (Purple Sea Urchin) Embryo (Cat no:66851-1-Ig)

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody primarily labeled a band around 110 kD; by IF, it cannot label the ciliary localisation of Smo when the cells were stimulated for Hh pathway.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF